Growth of Bacterial Organisms: In Vitro Inhibition of Bacterial Growth Using Different Dental Adhesive Systems

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This study evaluated the antibacterial potential of four different adhesive systems.

Materials and Methods:

The adhesives used in this study were Gluma Comfort Bond + Desensitizer (Heraeus Kulzer, Hanau Germany), Gluma Comfort Bond (Heraeus Kulzer), both of which are etch-and-rinse adhesives, and iBond (Heraeus Kulzer) and One-Up Bond F (Tokuyama, Tokyo, Japan), which are self-etching adhesives. Glutaraldehyde is present in Gluma Comfort Bond + Desensitizer and iBond. The bonding systems were applied to 6.5-mm paper discs, the solvents were evaporated, and the adhesives were light-activated for 20 seconds using a halogen curing unit.

Materials and Methods:

Four species of bacteria were tested: Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, and Actinomyces viscosus. The bacteria were cultured, and suspensions were prepared and placed on agar plates. The specimens were placed on the freshly inoculated agar plates for initial values and then were tested at 1 week, 1 month, 3 months, and 6 months of storage. Each plate contained five discs, one of each adhesive group plus a blank disc. The zone of bacterial inhibition of growth was measured using callipers.

Materials and Methods:

Bactericidal activity was tested with measured cultures of the bacteria placed on the surface of the cured adhesive discs or a blank disc. The bacteria were recovered after 1 hour, and the number of recovered viable bacterial determined by counting colonies of dilutions. A 100-fold reduction in recoverable CFU/mL compared with the blank disc was interpreted as significant killing.


The bacterial assays showed that all of the tested materials were capable of killing the test strains at the initial time. All of the materials inhibited development of bacterial growth immediately under the disk even when aged to 6 months. The inhibition of bacterial growth noted with iBond, especially against mutans streptococci, tended to be greater than the others.


The assays revealed potentially important differences in antimicrobial properties of distinct formulations of dental adhesives. The tested materials had a potential effect against representative oral plaque bacteria with cariogenic potential. This effect was demonstrated to be long-lasting in the in vitro simulation. iBond (which contains 4-META, UDMA, glutaraldehyde, acetone, and water) was the only adhesive system tested that was able to kill all the bacteria through at least 1 week of aging.

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