Identification of mRNA of the Inflammation-associated Proteins CXCL8, CXCR2, CXCL10, CXCR3, and β-Arrestin-2 in Equine Wounded Cutaneous Tissue: a Preliminary Study


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Abstract

Horses often sustain cutaneous wounds and healing can be prolonged and difficult to treat. Compared to body wounds, limb wounds heal slower and are more likely to develop exuberant granulation tissue. Differences in healing rates and exuberant granulation tissue formation is attributed to abnormal cytokine profiles. CXCL8 and its receptor CXCR2 are involved in acute inflammation whereas CXCL10 and its receptor CXCR3 are involved in inflammation resolution. β- arrestin-2 regulates inflammation through internalization of G-protein coupled receptors (GPCRs) including CXCR2 and CXCR3. Gene expression of these five inflammation associated proteins have not been previously identified in equine cutaneous tissue and may play a role in dysregulation of inflammation in equine limb wounds. The mRNA expression levels were measured using QuantiGene Plex Assay from cutaneous biopsies collected from surgically created wounds on the limb and thorax on days 0, 1, 2, 7, 14, and 33 from two horses. The mRNA expression levels were measured in mean fluorescent intensity and graphed. We were successful in identifying all five proteins for the first time in equine cutaneous tissue. Preliminary results suggest that there are different expression patterns for CXCL8, CXCR2 and β-arrestin-2 between the limb and thorax but not for CXCL10 and CXCR3. Differential regulation of CXCL8, CXCR2 and β-arrestin-2 may further explain why limb wounds heal differently than body wounds and warrants further investigation.HighlightsCXCL8, CXCR2, CXCL10, CXCR3 and β-arrestin-2 were identified in equine cutaneous tissue.CXCL8, CXCR2, and β-arrestin-2 expression patterns are different between limb and body wounds.Differential expression patterns further explain slowed and complicated healing of limb wounds.

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