[PS 01-03] Apolipoprotein E genotype polymorphism as a factor in determination of the dyslipidemia type in men with essential hypertension, residents of Podillja region of Ukraine

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Abstract

Objective:

Apolipoprotein E (ApoE) is a major part of most lipoproteins and is involved in cholesterol transport. Therefore, its polymorphism may contribute to development of dyslipidemia. The aim of the study is to determine the correlation of lipid spectrum variants in the blood plasma in healthy men and patients with essential hypertension (EH), residents of Podillja region of Ukraine, who has different ApoE genotypes.

Design and Method:

The study involved 79 healthy men aged 40–60 years (49,01 ± 0.73 years) and 112 patients with essential hypertension (EH), mean age (49,61 ± 0,73 years). Genotyping was conducted using polymerase chain reaction. Determination of the lipid spectrum was performed on biochemical analyzer according to enzymatic colorimetric method.

Results:

From the 6 possible genotypes only 3 occurred in this population: E2E3, E3E3, E3E4. Frequency distribution of genotypes in patients with EH had no significant differences from those in healthy men of the same age (p ≥0.05), with the most frequent one being E3E3. In healthy men modifications of lipid spectrum correlated within the accepted norms of blood lipoprotein concentrations, at the same time higher lipoprotein levels (LDL cholesterol, VLDL cholesterol) were in men with genotypes E3E3 and E3E4. In patients with EH, more pronounced proatherogenic changes in lipid spectrum were determined in patients with E3E3, E3E4 genotype: the concentations of LDL cholesterol (3,99 ± 0,09; 4,14 ± 0,26 mmol /l) and VLDL cholesterol (1,68 ± 0,03; 1,59 ± 0,1 mmol /l) were significantly higher, the concentations of HDL cholesterol (0,99 ± 0,03; 1,01 ± 0,08 mmol /l) were significantly lower, respectively, in comparison with those who had E2E3 genotype (p < 0.05).

Conclusions:

The frequency of occurrence of certain ApoE genotypes in men living in Podillja region of Ukraine corresponds to the European population. In patients with EH, who had E3E4, E3E3 genotypes shifts in the spectrum of lipids can be expected corresponding to Fredrickson dyslipidemia of the 2nd class.

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