To examine the impact of plasmapheresis on the level of inflammatory cytokines and markers of endothelial dysfunction in patients with essential hypertension (EH).Design and Method:
We studied on 34 patients with hypertension aged 32 to 54 years (mean age 50.3 ± 2.03y): including 25 men and 9 women (mean EH duration 9.1 ± 7y). We measured interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), endothelin-1 (En-1) in all patients, before and after the three procedures of plasmapheresis within two weeks period. Interleukin-6 (IL-6), tumor necrosis factor α (TNF- α), endothelin-1 (ET-1) were measured by immunoenzyme assay.Results:
The content of anti-inflammatory cytokines in the blood serum (IL-6, and TNF) of patients with EH was significantly lower after plasmapheresis and were respectively as follow: before- IL-6 - 164.6 ± 14.2 pg/mL, after 135.2 ± 11.7 pg/mL (p < 0.05), before TNF-α – 204.3 ± 10.5 pg/mL and after 167.6 ± 12.4 pg/mL (p < 0.05). The correlation was detected between the level of TNF-α and duration of EH (r = 0.42, p = 0.002) and the stage of EH (r = 0.5, p = 0.007). We found another correlation between the level of IL-6 and duration of hypertension (r = 0.56, p = 0.004). The Level of ET-1 after plasmapheresis was significantly lower than the baseline value, and were as follow, 0.21 ± 0.02 fmol/mL versus 4.24 ± 0.20 fmol/mL, p < 0.05. We found a correlation between the level of ET-1 and duration of essent ial hypertension (r = 0.56, p = 0.003).Conclusions:
After the plasmapheresis in patients with essential hypertension, there was a significant alteration in level of inflammatory cytokines and markers of endothelial dysfunction. These changes were indicating the improvement of endothelial function.