Vascular endothelial growth factor (VEGF) is an important regulator of vasculogenesis and plays a critical role in development of cerevrovascular and cardiovascular disease like stroke. VEGF single nucleotide polymorphisms (SNPs) −2578C>A,−1154G>A, and +936C>T have been associated with VEGF expression and production, however their effects on the development with the stroke in patients with hypertension have not been studied well separately. Therefore, this study was designed to investigate the association of VEGF polymorphism with stroke in patients with hypertension.Design and Method:
Real-time PCR combined with melting curve analysis in LightCyler was used for the amplification and genotyping of VEGF SNPs.Results:
Among the three studied SNPs of VEGF, a significant differences in the allele and genotype frequencies were found only with VEGF −2578C>A between the stroke with Hypertension and stroke without hypertension (p = 0.003). Compared with the −2578CC genotype, the CA genotype and CA+AA (A-allele) increased the risk of stroke development in hypertensive patients with the odd ratio (OR) 2.33 (95%CI = 1.42–3.81), p = 0.0007 and 2.28 (95%CI = 1.41–3.69), p = 0.0006), respectively.Conclusions:
This study suggests first time the genetic variation in VEGF gene might contribute to the development of stroke specially in hypertensive patients.