PS 07-25 CHANGES OF CASPASE-3, BAX, Bcl-2, CHEMOKINE RECEPTOR-2, MONOCYTE CHMOATTRACTANT PROTEIN-1, AND TRANSFORMING GROWTH FACTOR β GENES IN SPONTANEOUSLY HYPERTENSIVE RAT AFTER LOSARTAN TREATMENT

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Abstract

Objective:

Increased apoptosis has been demonstrated recently in the hypertrophied left ventricle of spontaneously hypertensive rat (SHR). Although the available evidence suggests that apoptosis can be induced in cardiac cells by a variety of insults including pressure overload, it appears that cardiac apoptosis in adult SHR results from an exaggerated local production of angiotensin.

Objective:

The changes in pathology and six gene expressions such as caspase-3, Bax, Bcl-2, chemokine receptor (CCR)-2, monocyte chmoattractant protein (MCP)-1and transforming growth factor (TGF) β were investigated to explore the effects of losartan in SHR.

Design and Method:

Twelve week-old male Wistar rats were grouped as follows: control (C) group, hypertension (H) group and losartan (L) group in which SHR was treated with losartan (10 mg/kg/day) for 5 weeks. Western blot and reverse transcription polymerase chain reaction analysis were performed.

Results:

Systolic blood pressure was significantly decreased in the L group compared with the H group in weeks 3 and 5.

Results:

Caspase-3, Bcl-2, CCR-2, MCP-1 and TGF β genes were significantly increased in the H group compared with the C group in weeks 3 and 5. Caspase-3, MCP-1 and CCR-2 genes were significantly decreased in the L group compared with the H group in week 5. Bcl-2 was significantly decreased in the L group compared with the H group in week 3.

Conclusions:

Losartan reduced caspase-3, MCP-1, CCR-2 and Bcl-2 gene expressions. Losartan decreased inflammation and apoptosis. Further study is needed for differing doses of losartan in SHR models.

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