PS 10-28 ULMUS DAVIDIANA ETHANOL EXTRACT PREVENTS MONOCYTE ADHESION TO TNF-α-STIMULATED ENDOTHELIAL CELLS VIA INHIBITION OF VASCULAR CELL ADHESION MOLECULE-1

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Abstract

Objective:

Ulmus davidiana var. japonica Rehder (UD) has long been used for anticancer and anti-inflammatory therapy as in traditional Korean medicine. This study was designed to investigate the effect of UD on tumor necrosis factor-α (TNF-α)-induced monocyte adhesion to endothelial cells and its underlying mechanism.

Design and Method:

The anti-adhesive activities of UDE on monocyte-endothelial cell adhesion, vascular cell adhesion molecule (VCAM)-1 expression, reactive oxygen species (ROS) production, and nuclear factor (NF)-κB activity were investigated in tumor necrosis factor (TNF)-α-stimulated human umbilical vein endothelial cells (HUVEC).

Results:

UDE (3 - 30 μg/mL) showed no cytotoxicity, but significantly blocked TNF-α-induced monocyte-endothelial cell adhesion, VCAM-1 expression, and luciferase activity of the VCAM-1 promoter. Additionally, TNF-α-induced ROS generation, NF-κB nuclear translocation, and IκBα degradation were effectively inhibited by treatment with 30 μg/mL of UDE in HUVECs.

Conclusions:

Taken together, we demonstrated that UDE treatment inhibits TNF-α-induced monocyte adhesion to endothelial cells, suggesting that UD may reduce vascular endothelial inflammation.

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