Caloric restriction (CR) is effective for slowing aging and delaying aging-related diseases in many species, but the mechanism is complex and not fully elucidated. This study aimed to evaluate the beneficial effects of a caloric restriction diet on learning memory and to elucidate the mechanisms.Design and method:
Thirty 6-week-old male C57/BL mice were randomly divided into three groups: normal control (NC group), high-energy (HE group) and CR. After 10 months, the Morris water maze was used to examine learning and memory abilities. Western blotting and immunohistochemistry were used to detect changes in proteins involved in Silent information regulator 2 related enzyme 1 (SIRT1)/AMP-activated protein kinase (AMPK) pathway in the mouse hippocampus.Results:
Compared with the NC group, the average escape latency and swimming distance were lower in the CR group. The protein expression of SIRT1 and AMPK in the CR group was significantly higher than that in the control and HE groups.Conclusions:
Our findings demonstrate that a CR diet may improve hippocampus-dependent spatial learning ability of C57/BL mice, accompanied with an increase in SIRT1 and AMPK expression. We showed for the first time that CR could improve learning and memory, probably via regulation of the SIRT1/AMPK signaling pathway.