[PP.LB02.14] RENAL DENERVATION DECREASES NA+/CL- CO-TRANSPORTER (NCC) IN GOLDBLATT MODEL OF HYPERTENSION

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Abstract

Objective:

It is known that increased renal sympathetic nerve activity (RSNA) has an important role in the development and maintenance of renovascular hypertension (2K1C). In 2K1C model, both kidneys, clipped (C) and non-clipped (NC), acts to maintain sodium and water balance. However, the effects of RSNA on sodium renal transporters and renal function remain unclear. The aim of this study was evaluate the effects of renal denervation (RDN) on glomerular filtration rate (GFR), urinay flow (UF) and (NCC) protein expression in C and NC kidneys.

Design and method:

A silver clip was implanted in the left renal artery in male Wistar rats and four weeks after clipping, the clipped kidney was denervated (all visible bundles nerves were dissected and renal vessels were painted with 10% phenol). All experiments were performed 2 weeks after RDN.

Results:

The GFR was decreased in C and NC kidney in 2K1C rats compared to control group (Ctr), RDN didn’t change GFR (Non-clipped kidney: Ctr 0,4 ± 0,09; 2K1C 0,2 ± 0,02; 2K1C RDN 0,2 ± 0,07; Clipped kidney: Ctr: 0,6 ± 0,1; 2K1C 0,14 ± 0,02; 2K1C RDN: 0,25 ± 0,06 mL/min). In NC kidney, UF was increased compared to Ctr and was significantly decreased after RDN (Ctr 0,003 ± 0,001; 2K1C: 0,014 ± 0,003; 2R1C RDN: 0,006 ± 0,001 mL/min). NCC protein expression was increased in both C and NC kidneys in 2K1C rats and RDN decreased NCC protein expression only in NC kidneys (Non-clipped kidney: Ctr 95 ± 6; 2K1C 144 ± 8; 2K1C DNX 60 ± 15; Clipped kidney: Ctr: 100 ± 10; 2K1C: 158 ± 15; 2K1C RDN: 140 ± 16).

Conclusions:

Altogether, the results suggest that RSNA has an important role in the NCC protein expression in NC kidney in 2K1C independent of renal function parameters. Financial Support: CAPES, CNPq and FAPESP.

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