28 GREEN TEA EXTRACT ADMINISTRATION HAD A BENEFICIAL EFFECT ON PPAR ALPHA AND PPAR GAMMA GENE EXPRESSION IN METABOLIC SYNDROME RAT MODEL

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Abstract

Background:

PPARs play important role in metabolic syndrome regulation thus, they become the molecular therapeutic target of some drugs. The benefit of green tea extract administration on these molecular targets needs further investigation. This study aimed to investigate the effect of green tea extract administration on PPARα and PPARγ gene expression in metabolic syndrome rat models.

Methods:

Thirty Sprague Dawley Metabolic Syndrome Rat Model weighed 300 – 400 grams were divided into EGCG 200 (n = 5), EGCG 300 (n = 5), and EGCG 400 (n = 5) groups. Moreover, as control groups fifteen rats were divided into normal control (NC) (n = 5), simvastatin and metformin treated (SM) (n = 5), and metabolic syndrome (MS) (n = 5) groups. Rats in the EGCG 200, 300, and 400 groups were treated once daily with green tea extract at a dose of 200, 300, and 400 mg/bw.t respectively. Moreover, Rats in the SM group were treated daily with 10 mg/bw.t of simvastatin and 200 mg/bw.t of metformin. The extract as well as the drugs were administrated for 9 weeks through oral gavage. Fasting blood glucose, triglyceride, and HDL level was measured every two weeks by colorimetric method. Blood pressure was determined by tail cuff blood pressure recorder. PPARα and PPARγ gene expression was analysed by RT-PCR methods.

Result:

After administration of green tea extract for 9 weeks, fasting blood glucose, triglyceride, and systolic blood pressure were significantly lower (p < 0.05), and HDL-Cholesterol was significantly higher in all EGCG groups compared to that of MS group. Moreover, PPARα gene expression significantly higher in all EGCG and SM group (p > 0.05) after 9 week compared to that of MS group. Moreover, PPARα gene expression in all EGCG group were similar with that of SM groups (p > 0.05). Furthermore, PPARγ gene expression were significantly higher in all EGCG group compared to that of MS groups (p < 0.05). On the other hand, PPARγ gene expression in SM group were significantly higher (p < 0.05) compared to those of all EGCG group.

Conclusion:

Green tea extract administration for 9 weeks have beneficial effect in increasing gene expression of PPARα and PPARγ in metabolic syndrome rat model

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