30 GREEN COFFEE EXTRACT INCREASED PPAR ALPHA AND PPAR GAMMA GENE EXPRESSION IN METABOLIC SYNDROME RAT MODEL

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Abstract

Background:

PPARs act as pivotal regulators of metabolic syndrome. PPARα and PPARγ are the molecular targets of a number of marketed drugs for the treatment of metabolic syndrome. Green coffee administration is a new candidates of metabolic syndrome treatment which need further investigation of its mechanism. This study aimed to investigate the effect of green coffee extract administration on PPARα and PPARγ gene expression in metabolic syndrome rat models.

Methods:

Thirty Sprague Dawley Metabolic Syndrome Rat Model weighed 300 – 400 grams were divided into CGA 100 (n = 5), CGA 200 (n = 5), and CGA 250 (n = 5) groups. Moreover, as control groups fifteen rats were divided into normal control (NC) (n = 5), simvastatin and metformin treated (SM) (n = 5), and metabolic syndrome (MS) (n = 5) groups. Rats in the 100 groups were treated once daily with green coffee extract at a dose of 100, 200, and 250 mg/bw.t respectively. The extract was given for 9 weeks through oral gavage. Fasting blood glucose, triglyceride, and HDL level was measured every two weeks by colorimetric method. Blood pressure was determined by tail cuff blood pressure recorder. PPARα and PPARγ gene expression was analyzed by RT-PCR methods.

Result:

Fasting blood glucose, triglyceride, and systolic blood pressure significantly decreased (p < 0.05) and HDL-Cholesterol was significantly higher in all CGA groups after administration of green coffee extract for 9 weeks. Moreover, after administration of green coffee extract for 9 weeks, PPARα gene expression was significantly higher in all CGA and SM group (p > 0.05) compared to that of MS group. Furthermore, PPARα gene expression in CGA 100 and 200 group were not significantly different (p > 0.05) compared to that of SM group. Furthermore, PPARγ gene expression in all CGA group showed significantly higher expression compared to that of MS group(p < 0.05). On the other hand, PPARγ gene expression in SM group were significantly higher (p < 0.05) compared to that all CGA group after 9 week

Conclusion:

Administration of green coffe extract for 9 weeks have beneficial effect in improvement of PPARα and PPARγ gene expression in metabolic syndrome rat model

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