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To evaluate the difference of hypertension prevalence between blood pressure(BP) measurement in first day or 3 different days in community population.47 955 residences aged 35–75 years old (54.25 ± 15.28y)in Provence of Zhejiang, Henan and Beijing City were included. Blood pressure (BP) was measured for three times with one minute interval and averaged as the final values. Suspected hypertension was defined as systolic BP (SBP)≥140 mm Hg and/or diastolic BP (DBP)≥90 mmHg in the survey, without diagnosed hypertension. Patients with suspected hypertension received BP measurement in other two days within one month. Clinical hypertension was defined that all three BP readings in different days fulfill the above-mentioned standard. Effect on hypertension diagnosis was contrasted between BP measured in First Day or 3 Different Days.There were 7344 diagnosed hypertension patients and 3221 suspected hypertension patients, and hypertension prevalence was 22.03% by BP measurement in first day. Among 3221 patients with suspected hypertension, only 425 (13.19%)were diagnosed as clinical hypertension by BP measurement in 3 different days. Thus, the prevalence of clinical hypertension was 16.20%, 5.83 percentages lower than that by measured in first day. There was significant difference in age, heavy drinking and first-day SBP between hypertension or non-hypertension diagnosed from 3221 suspected hypertension by 3-different-days measurement. Logistic regression analysis showed 65–74yr(OR = 4.470), ≥75yr(OR = 5.358) increased the risk of hypertension diagnosis by 3-different-days measurement, high salt diet(OR = 0.331) decreased the risk.Hypertension prevalence would be overestimated by BP measured in first day, compare with in 3 different days.