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To summarize the rhythm variation of 24-hour ambulatory blood pressure (ABP) between Chinese male shift workers and day workers.176 subjects, including 77 shifters and 99 day workers, were collected information on clinical characteristics and laboratory data, performed measurements of ABP. Day-nocturnal and awake-sleep rhythm variation of ABP were compared between groups.24-hour ABP was similar in two groups. Day-nocturnal rhythm variation was disappeared among the shifters, while existed among the day workers, showed a statistically significant difference, even after adjustment for co-variables (P = 0.0008). Whereas, awake-sleep rhythm was both existed among two groups, but the group of day workers was higher (P < 0.01).Rhythm variation of 24-hour ambulatory blood pressure among shifters was along with awake-sleep pattern, rather than day-nocturnal one.