24 Danggui shaoyao powder improves diabetic nephropathy without altering blood glucose in streptozotocin-induced diabetic rats

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Abstract

Objectives

Diabetic nephropathy (DN) is one of the most severe diabetic complications and is becoming a worldwide epidemic, accounting for approximately one-third of all cases of end-stage renal disease. In this study, we investigated its influence on Th1/Th2 cells balance, inflammation and lipid metabolism to assess whether Danggui Shaoyao Powder (DSS) ameliorates DN.

Methods

Wistar rats were randomly divided into control group, model group, DSS group and benazepril group. The model group was induced by injection intraperitoneally with streptozotocin after high-calorie foods were given for 1 month. Animals were treated orally with saline, DSS and benazepril daily for 8 weeks. At the end of 8 weeks, blood glucose, 24 hour urinary protein (24 hour UPr), creatinine clearance rate (Ccr), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), serum levels of interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, interleukin (IL)−4, and IL-10 were measured. Glomerular morphology was observed by light microscopy.

Results

Compared with rats in the control group, rats in the DN group had significantly increased 24 hour UPr, Scr, blood lipids and pro-inflammatory cytokines IFN-gamma and TNF-alpha secreted by Th1 cells. In addition, down-regulation of the proportion of Th2 cells and decrease of the anti-inflammatory cytokines IL-4 and IL-10 secreted by Th2 cells occurred. Compared with the model group, the DSS group had significantly decreased levels of 24 hour UPr, Scr, TC, TG, LDL, IFN-gamma and TNF-alpha, and increased IL-4 and IL-10. The renal pathological changes in the DSS treatment group were ameliorated.

Conclusion

This study suggests that DSS could improve renal function in streptozotocin-induced DN model rats. The mechanism may be related to regulating the Th1/Th2 cell balance and improved lipid metabolism in DN rats.

Acknowledgment

Supported by a project grant from The National Natural Science Foundation of China (Grant No. 81603527), Science and technology project of Henan Province (Grant No. 162102310466), Key scientific research projects of Henan Province Colleges and Universities (Grant No. 16A360010), Henan University of Traditional Chinese Medicine Scientific and technological innovation talent support program (Grant No. 2015XCXRC05), Science and technology project of Zhengzhou City (Grant No. 20150310).

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