Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections.Methodology.
This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae.Results.
DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 μmol l−1, and minimum bactericidal concentration ranging between 69 and 277 μmol l−1. It significantly reduced bacterial biofilm survival - by 22-63% (at MIC, 10×MIC or 100×MIC) as compared to the 25-42% reduction by vancomycin (P<0.0001) - and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations.Conclusion.
These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity.