Nonhuman primates are of interest as models of human physiology to study the effect of multiple pregnancies on birth weight. Reference plasma and red blood cell (RBC) total phospholipids fatty acids were established in nonpregnant breeding female Vervet monkeys. Twentythree clinically healthy nonpregnant Vervet monkeys (Cercopithecus aethiops), contained in a controlled closed environment and consuming a high carbohydrate diet (68 E%) that contained 20 E% fat and 12 E% protein were sampled for blood during a cross-sectional study. A low intake of ω3 fatty acids was reflected by a high ω6/ω3 ratio (66:1) of the diet. Inverse relations were seen between plasma and RBC total phospholipid fatty acids, 18:2ω6, 20:3ω6, and 20:4ω6, which suggested selective incorporation in membranes. Low levels of 20:5ω3 and 22:6ω3 of plasma and RBC total phospholipids render Vervet monkeys as ideal subjects to study the effect of ω3 fatty acid supplementation on pregnancy outcomes.