Epri to Estimate the in Vivo Intracerebral Reducing Ability in Adolescent Rats Subjected to Neonatal Isolation

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To estimate the in vivo intracerebral reducing ability after acute stress in adolescent rats subjected to early neonatal isolation (NI), by performing temporal electron paramagnetic resonance imaging (EPRI) of the brain.

Materials and Methods:

An EPRI system operating at an EPR frequency of 700 MHz was used. The intracerebral reducing ability was estimated based on the halflife of the EPR signal of the blood–brain barrier (BBB)-permeable nitroxide radical. The NI treatment was performed for a period of one hour per day over postnatal days 2–9. Six-week-old rats were exposed to acute stress (immobilization for 90 minutes) prior to the EPRI study.


Depletion of the intracerebral reducing ability caused by the acute stress was observed; however, this depletion phenomenon did not occur in animals that were not subjected to NI.


The results obtained in this study prove that NI induces cerebral vulnerability to acute stress in adolescence.

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