Sporotix Schenckiiis a pathogenic fungus that causes human and animal sporotrichosis, and based on morphology of the sessile conidia and molecular analysis, it was recently recognized as a species complex comprising at least the following six sibling species:S. albicans,S. brasiliensis,S. globosa,S. luriei, S. mexicanaandS. schenckii. However, apart fromS. schenckiisensu strict, onlyS. brasiliensis,S. globosaandS. lurieiare associated with human and animal infection.S. globosahas been most commonly isolated in Asia, Europe and the USA; therefore, molecular epidemiological study forS. globosais important in relation to human sporotrichosis in Japan. To the best of our knowledge, this is the first study to determine the mating type 1–2 (MAT1–2) gene ofSporothrix schenckiiwith the aim of understanding the taxonomy of the genusSporothrix. TheMAT1–2gene (1618 bp) encodes a protein sequence of 198 amino acids. Reverse transcription polymerase chain reaction analysis also detectedMAT1–2gene mRNA expression in all of theS. schenckiistrains examined, indicating that this gene is expressed inS. schenckiicells. Phylogenetic analysis of theMAT1–2gene fragments ofOphiostoma himal-ulmi,O. novo-ulmi,O. ulmiandS. schenckiiindicated that these isolates could be classified into four clusters.MAT1–1gene-specific polymerase chain reaction was positive in 15 isolates, but negative in four human isolates and one feline isolate.