Immune Responses to Recombinant Proteins ofMycobacterium leprae

    loading  Checking for direct PDF access through Ovid

Abstract

Identification of antigenic determinants of the polar immune response in leprosy may illuminate both protection and pathogenesis. Thirty subjects were studied (22 with polar disease and 8 healthy controls who were heavily exposed but disease-free) by assaying the proliferative, interferon (IFN)-γ, and antibody responses to recombinant antigens of Mycobacterium leprae (10, 28, 36, and 65 kDa). The 10-kDa antigen elicited IFN-γ production from all tuberculoid (TT) and borderline tuberculoid (BT) patients but little from controls, lepromatous (LL), or borderline lepromatous (BL) patients (P < .05) Production of 65-kDa-specific IFN-γ was higher in TT/BT than in controls or LL/BL patients (P < .006). All subjects produced 65-kDa-specific antibody, but it was higher in LL/BL patients than in healthy controls, whose responses were higher than in TT/BT subjects (P = .035). The 36-kDa antibody responses were selectively increased in LL/BL subjects (P < .02). The intermediate phenotype of the controls suggests that M. leprae-specific production of IFN-γ may contribute to pathology and to protection in leprosy.

Related Topics

    loading  Loading Related Articles