Hepatitis E virus sequences were detected by RT-PCR in 14/15 (93%) of untreated sewage samples from Edinburgh, Scotland, UK. Phylogenetic analysis of amplicons at limiting dilution revealed the co-circulation of multiple variants of HEV-3, with a pattern of diversity matching that observed in a local cohort of HEV-infected hepatitis patients. J. Med. Virol. 88:915–918, 2016. © 2015 The Authors. Journal of Medical Virology Published by Wiley Periodicals, Inc.