Microbiological Composition Associated With Interleukin-1 Gene Polymorphism in Subjects Undergoing Supportive Periodontal Therapy

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Abstract

Background:

Interleukin-1 gene polymorphism (IL-1 gene) has been associated with periodontitis. The present study examined the subgingival microbiota by IL-1 gene status in subjects undergoing supportive periodontal therapy (SPT).

Methods:

A total of 151 subjects with known IL-1 gene status (IL-1A +4845/IL-1B -3954) (IL-1 gene) were included in this study. Clinical data and subgingival plaque samples (40 taxa) were collected. These taxa were determined by the checkerboard DNA-DNA hybridization method.

Results:

Gender, smoking habits (n-par tests), age, and clinical periodontal conditions did not differ by IL-1 gene status. IL-1 gene-negative subjects had a higher total bacterial load (mean difference, 480.4 × 105; 95% confidence interval [CI], 77 to 884 × 105; P <0.02). The levels of Actinobacillus actinomycetemcomitans (mean difference, 30.7 × 105; 95% CI, 2.2 to 59.5 × 105; P <0.05), Eubacterium nodatum (mean difference, 4.2 × 105; 95% CI, 0.6 to 7.8 × 105; P <0.02), Porphyromonas gingivalis (mean difference, 17.9 × 105; 95% CI, 1.2 to 34.5 × 105; P <0.05), and Streptococcus anginosus (mean difference, 4.0 × 105; 95% CI, 0.2 to 7.2 × 105; P <0.05) were higher in IL-1 gene-negative subjects, an observation specifically found at sites with probing depths <5.0 mm.

Conclusions:

Bleeding on probing did not differ by IL gene status, reflecting clinical SPT efficacy. IL-1 gene-negative subjects had higher levels of periodontal pathogens. This may suggest that among subjects undergoing SPT, a lower bacterial load is required in IL-1 gene-positive subjects to develop the same level of periodontitis as in IL-1 gene-negative subjects. J Periodontol 2006;77:1397-1402.

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