In a detailed study of the relation between the deflection caused by shear force and the constitution of a laminated material beam, we derived an equation for calculating the shear modulus of a laminated material beam from the shear moduli of individual laminae. The validity of the derived equation was investigated using crosslaminated wood beams made with five species. The calculated shear moduli parallel to the grain of face laminae ranged from 48.3 MPa to 351 MPa, while those perpendicular to the grain of face laminae ranged from 58.0 MPa to 350 MPa. The calculated shear moduli increased markedly with increasing shear modulus in a cross section of perpendicular-direction lamina of a cross-laminated wood beam. The calculated apparent modulus of elasticity (MOE) of cross-laminated wood beams agreed fairly well with the measured apparent MOE values. This fact indicated that the apparent MOE of cross-laminated wood beam was able to be calculated from the true MOE values and shear moduli of individual laminae. The percentage of deflection caused by shear force obtained from the calculated apparent MOE (Ysc) was close to that obtained from the measured apparent MOE (Ys) and there was a high correlation between both values. From the above results, it was concluded that the derived equation had high validity in calculation of shear modulus of a cross-laminated wood beam.