The importance of vial composition in HPLC analysis: An unusual case of phosphorous pseudorotation

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HIGHLIGHTSAlkalinity of glass is found to be a root cause for variable solution stability.Discrepancies in degradation between vendors indicate that manufacturing differences generate USP Type 1 vials with variable alkalinity.Possible remediations to proactively mitigate impact on analysis are discussed.Glass HPLC vials are ubiquitous in analytical laboratories and vendors have developed many varieties to meet the various needs of scientists. As such there may be multiple types of vials being used simultaneously in a single laboratory without much consideration as to which is best suited for analytical method development and validation. This study highlights the possibility of vial composition as a potential factor that impacts solution stability. Here we describe a case where the type of HPLC vial used results in an interesting phosphorous pseudorotation driven by the mild alkalinity of glass.

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