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Vitamin analysis by capillary electrophoresis reviewed from mid-2007 until mid-2017.Overview of electrophoretic modes, detection and sample concentration approaches.Table summary of pharmaceutical, dietary supplement and biological applications.Vitamins are essential molecules required for human metabolism such as energy production, immune system and cell formation. Accurate vitamin analysis is critical for disease diagnosis, nutrients assessment, as well as food and drug production. This review gives an overview of the recent developments in the use of capillary electrophoresis combined with different detection techniques for the analysis of vitamins. The period covers mid-2007 until mid-2017. Different separation modes are discussed for the analysis of water-soluble vitamins and fat-soluble vitamins. On-line sample concentration for sensitive analysis is also described. The applications include the analysis of pharmaceutical, dietary supplement and biological samples.