Investigation of the effect of blood hematocrit and lipid content on the blood volume deposited by a disposable dried blood spot collection device

    loading  Checking for direct PDF access through Ovid


Graphical abstractHighlightsInnovative disposable volumetric dried blood sampler tested.Consistent blood volumes derived regardless of hematocrit, or lipid content.Volumetric performance was consistent with that of a pipette.Device was simple to use and gave 92.9% acceptable samples.When using dried blood spot (DBS) sampling for the quantitative bioanalysis of circulating concentrations of drugs, metabolites and endogenous analytes, it is important that a fixed volume of blood is deposited to overcome the issues associated with blood hematocrit (HCT) and homogeneity. The volumetric performance of the KTH DBS collection device was tested with radiolabelled [14C]-diclofenac. It was demonstrated that the device deposits a fixed volume of blood (13.5 μL) regardless of the HCT (25–65%), or lipid content of the blood sample. Further, it was found that the precision and accuracy of the derived dried blood samples were at least as good as those of a positive displacement pipette. The device was found to be easy to use and gave acceptable dried samples for 92.9% of the tests performed (n = 42).

    loading  Loading Related Articles