Determination of docetaxel in dried blood spots by LC–MS/MS: Method development, validation and clinical application


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Abstract

HIGHLIGHTSDocetaxel can be measured in dried blood spots by LC–MS/MS.Docetaxel plasma concentrations can be estimated through DBS measurements.Area under the curve (AUC) are accurately obtained from DBS concentrations.Docetaxel measurement in DBS can potentially be used to optimize cancer therapy.In this study, a LC–MS/MS method for the measurement of docetaxel in Dried Blood Spots (DBS) samples was developed and validated. Docetaxel was extracted from 8mm DBS punch with a mixture of methanol and acetonitrile (9:1, v/v). The chromatographic separation occurred in an Acquity® C18 column (150×2.1mm, 1.7μm) eluted with a mixture of water and acetonitrile plus 0.1% formic acid (45:55, v/v). Total analytical run time was 7min. The method was linear from 50 to 3000ngml−1. Precision assays showed CV%<9.8% and accuracy between 99 and 103%, mean recovery was 81%. The method was applied in the determination of the docetaxel in 31 patients, after collection of two paired venous blood and DBS samples, following a limited sampling strategy protocol. The analyte was stable in DBS for 18days at 25°C and 9days at 45°C. The interval of docetaxel concentrations measured in DBS collected before the end of the infusion was 756–3047ngml−1 and 60±10min after the end of the infusion was 57–331ngml−1. AUC values calculated from DBS-derived estimated plasma concentrations (EPC) represented on average 100% of those obtained in plasma samples of 3.1mgh/l (2.4–4.9mgh/l). There was a 93% agreement between the classification of patients as within or without the therapeutic range by plasma and EPC AUC. These findings support the clinical use of DBS sampling for routine therapeutic drug monitoring of docetaxel.

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