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Magnolosides were electrochemically determined in a two-channel LC.Chromatograms of magnolosides A, B, D, F, H, L and M were measured within 85min.Accurate and precise quantitative profiles of magnolosides were obtained.The profiles of magnolosides could be applied to a multivariable analysis.Magnoliae officinalis cortex were discriminated by the profiles of magnolosides.A two-channel liquid chromatography with electrochemical detection system (2LC-ECD) was newly designed for the simultaneous determination of magnolosides A, B, F, H, and L in the first channel and other magnolosides D and M in the second channel, respectively. Peak heights had linear relationships to the magnoloside concentrations in a range of 0.02–16μmol/L for H, 0.01–12μmol/L for A, 0.02–12μmol/L for F and L, 0.01–8μmol/L for B, 0.002–6μmol/L for D, and 0.002–4μmol/L for M, respectively. Seven magnolosides in magnoliae officinalis cortex (MOC) were determined by the 2LC-ECD, and the obtained quantitative profiles of magnolosides were applied to the discrimination between the MOC samples harvested from Hubei and Sichuan (called Chuan po) and from Zhejiang and Fujian (called Wen po). By principal component analysis (PCA) and supervised partial least squares discriminant analysis (PLS-DA) based on the quantitative profiles of the magnolosides, Chuan po were clearly discriminated from Wen po on the plots obtained from our multivariable analyses.