Ouabain exerts cytoprotection by diminishing the intracellular K+ concentration increase caused by distinct stimuli in human leukemic cells

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Abstract

Objectives

We tested if modulation of cytosolic K+ levels by ouabain, an inhibitor of Na+/K+-ATPase, exerts cytoprotection against distinct stressful stimuli in human leukemic cells.

Methods

The cytosolic K+, Na+ or Ca2+ levels and the cytotoxicity were evaluated by flow cytometry.

Key findings

Various cytotoxic chemicals and ultraviolet irradiation induced cell death and increased intracellular concentrations of K+, Na+ or Ca2+. Ouabain reduced the cytotoxicity and the elevation of cytosolic levels of K+ but not those of Na+ or Ca2+.

Conclusions

Our data thus suggest that elevated cytosolic K+ levels are associated with the cytotoxicity in response to distinct stressful stimuli and that ouabain exerts cytoprotection most probably by regulating intracellular K+ levels.

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