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To develop an equation to predict maximal oxygen uptake (VO2max) based on the 6-minute walk test (6MWT) and body composition in healthy boys.Direct VO2max, 6-minute walk distance, and anthropometric characteristics were measured in 349 healthy boys (12.49 ± 2.72 years). Multiple regression analysis was used to generate VO2max prediction equations. Cross-validation of the VO2max prediction equations was assessed with predicted residual sum of squares statistics. Pearson correlation was used to assess the correlation between measured and predicted VO2max.Objectively measured VO2max had a significant correlation with demographic and 6MWT characteristics (R = 0.11-0.723, P < .01). Multiple regression analysis revealed the following VO2max prediction equation: VO2max (mL/kg/min) = 12.701 + (0.06 × 6-minute walk distance m) – (0.732 × body mass indexkg/m2) (R2 = 0.79, standard error of the estimate [SEE] = 2.91 mL/kg/min, %SEE = 6.9%). There was strong correlation between measured and predicted VO2max (r = 0.875, P < .001). Cross-validation revealed minimal shrinkage (R2p = 0.78 and predicted residual sum of squares SEE = 2.99 mL/kg/min).This study provides a relatively accurate and convenient VO2max prediction equation based on the 6MWT and body mass index in healthy boys. This model can be used for evaluation of cardiorespiratory fitness of boys in different settings.