Assuming velocity attainment, acceleration range of motion (AccROM) determines the amount of ROM spent under load range. This study sought to determine if gender, repetition type, and number affect knee extension AccROM. Subjects (15 M, 20 F; age 27.5 ± 6.5 yrs) performed 3 maximal concentric knee extension repetitions at isokinetic velocities between 60 and 450°/sec and 50 reps at 180°/sec. AccROM was defined as the ROM needed to attain the preselected velocity. The first repetition, starting from a dead stop, was identified as single while Repetitions 2 and 3 were identified as reciprocal. Results revealed that reciprocal repetitions had significantly (p < 0.05) less AccROM than single at all speeds. Men had significantly less AccROM than women during reciprocal repetitions at 120–450°/sec and during single reps at 240-450°/ sec. AccROM during 50 repetitions significantly increased every 10 reps from 20 through 50. To ensure maximum load range, knee extension exercise protocols on an isokinetic device should be specific to gender, repetition type, and number.