Ratamess, NA, Rosenberg, JG, Kang, J, Sundberg, S, Izer, KA, Levowsky, J, Rzeszutko, C, Ross, RE, and Faigenbaum, AD. Acute oxygen uptake and resistance exercise performance using different rest interval lengths: The influence of maximal aerobic capacity and exercise sequence. J Strength Cond Res 28(7): 1875–1888, 2014—The purpose of this study was to examine the relationship between V[Combining Dot Above]O2max and acute resistance exercise performance and the acute metabolic effects of exercise sequencing. Seventeen resistance-trained men were tested for V[Combining Dot Above]O2max and 1 repetition maximum (1RM) strength. Subjects were randomly assigned to either a group that performed the squat first in sequence followed by the bench press (S; n = 8) or a group that performed the bench press first followed by the squat (BP; n = 9). Each group performed 3 protocols (using 1-, 2-, or 3-minute rest intervals [RIs] between sets in random order) consisting of 5 sets of each exercise with 75% of their 1RM for up to 10 repetitions while oxygen consumption was measured. Total repetitions completed were highest with 3-minute RI and lowest with 1-minute RI. Mean V[Combining Dot Above]O2 was significantly highest with 1-minute RI and lowest using 3-minute RI. Analysis of each exercise revealed a tendency (p = 0.07) for mean bench press V[Combining Dot Above]O2 to be higher when it was performed after the squat using 1- and 2-minute RIs. V[Combining Dot Above]O2max was significantly negatively correlated to 1RM bench press and squat (r = −0.79 and −0.60, respectively) and was significantly correlated to squat repetitions (r = 0.43–0.57) but did not correlate to bench press performance. It seems that V[Combining Dot Above]O2max is related to lower-body resistance exercise performance when short RIs are used, and the metabolic response to the bench press is augmented when it follows the squat in sequence using short RIs.