Sánchez-Sánchez, J, García-Unanue, J, Felipe, JL, Jiménez-Reyes, P, Viejo-Romero, D, Gómez-López, M, Hernando, E, Burillo, P, and Gallardo, L. Physical and physiological responses of amateur football players on third generation artificial turf systems during simulated game situations. J Strength Cond Res 30(11): 3165–3177, 2016—The aim of this study is to evaluate the physical and physiological load imposed on amateur football players in a simulated game situation on different artificial turf systems. For that purpose, 20 football players (21.65 ± 3.10 year old) were monitored with Global Positioning Systems and heart rate bands during 45-minutes games on 4 selected artificial turf systems. The results show more covered distance in high-intensity ranges on the system with lower levels of damping and higher rates of rotational traction (p ≤ 0.05). Likewise, this system of artificial turf demonstrated a high number of sprints (12.65 ± 5.67) and more elevated maximum speed peaks during the last part of the game (28.16 ± 2.90 km·h−1) in contrast to the systems with better damping capacity (p ≤ 0.05). On the other hand, the physiological load was similar across the 4 artificial turf systems (p > 0.05). Finally, the regression analysis demonstrated a significant influence of the mechanical properties of the surface on global distance (15.4%), number (12.6%), and maximum speed (16.6%) of the sprints. To conclude, the mechanical variability of the artificial turf systems resulted in differences in the activity profiles and the players' perceptions during simulated football games.