Roche-Seruendo, LE, García-Pinillos, F, Haicaguerre, J, Bataller-Cervero, AV, Soto-Hermoso, VM, and Latorre-Román, PÁ. Lack of influence of muscular performance parameters on spatiotemporal adaptations with increased running velocity. J Strength Cond Res 32(2): 409–415, 2018—This study aimed to analyze the influence of muscular performance parameters on spatiotemporal gait characteristics during running when gradually increasing speed. Fifty-one recreationally trained male endurance runners (age, 28 ± 8 years) voluntarily participated in this study. Subjects performed a battery of jumping tests (squat jump, countermovement jump, and 20-cm drop jump), and after that, the subjects performed an incremental running test (10–20 km·h−1) on a motorized treadmill. Spatiotemporal parameters were measured using the OptoGait system. Cluster k-means analysis grouped subjects according to the jumping test performance, by obtaining a group of good jumpers (n = 19) and a group of bad jumpers (n = 32). With increased running velocity, contact time was shorter and flight time and step length were longer, whereas cadence and stride angle were greater (p < 0.001). No significant differences between groups (p ≥ 0.05) were found at any running speed. The results obtained indicate that increased running velocity produced no differences in spatiotemporal adaptations between those runners with good jumping ability and those with poor jumping ability. Based on that, it seems that muscular performance parameters do not play a key role in spatiotemporal adaptations experienced by recreational endurance runners with increased velocity. However, taken into consideration the well-known relationship between running performance and neuromuscular performance, the authors suggest that muscular performance parameters would be much more determinant in the presence of fatigue (exhausted condition) or in the case of considering other variables such as running economy or kinetic.