Wołyniec, W, Ratkowski, W, Kasprowicz, K, Jastrzębski, Z, Małgorzewicz, S, Witek, K, Grzywacz, T, Żmijewski, P, and Renke, M. Glomerular filtration rate is unchanged by ultramarathon. J Strength Cond Res 32(11): 3207–3215, 2018—Acute kidney injury (AKI) is reported as a common complication of marathon and ultramarathon running. In previous studies, AKI was diagnosed on the basis of the creatinine level in serum and estimated glomerular filtration rate (eGFR). In this study, we calculated eGFR and also measured creatinine clearance after every 25 km of a 100-km run. Twenty healthy, amateur runners (males, mean age 40.75 ± 7.15 years, mean body mass 76.87 ± 8.39 kg) took part in a 100-km run on a track. Blood and urine were collected before the run, after every 25 km, and 12 hours after the run. Seventeen runners completed the study. There was increase in creatinine, urea, and uric acid observed after 100 km (p < 0.05). The mean increase in creatinine was 0.21 mg·dl−1 (24.53%). Five runners fulfilled the AKI network criteria of AKI. The eGFR according to the modification of diet in renal disease, chronic kidney disease epidemiology collaboration, and Cockcroft-Gault formulas was significantly decreased after the run (p ≤ 0.05). Otherwise, creatinine clearance calculated from creatinine level in both serum and urine remained stable. In contrast to the majority of previous studies, we did not observe any decrease in the kidney function during an ultramarathon. In this study, the creatinine clearance, which is the best routine laboratory method to determine GFR was used. There is no evidence that long running is harmful for kidney.