EVIDENCE FOR A LOCAL ACTION OF MELATONIN ON THE RAT PROSTATE

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Abstract

Purpose

Melatonin, secreted by the pineal gland at night, inhibits pubertal development in rats and possibly humans. We have recently found functional specific binding sites for125 I-labeled melatonin (sup 125 I-melatonin) in human benign prostate tissue, localized in the microsomal fraction of the glandular epithelium. The aim of the present study was to set up an animal (rodent) model for the growth inhibitory effects of melatonin on the prostate.

Materials and Methods

Putative melatonin receptors in the rat ventral prostate were explored by means of autoradiography and receptor binding assays and the ability of melatonin to inhibit stimulated prostate growth was tested in vivo.

Results

In vitro autoradiography and equilibrium binding experiments demonstrated specific binding sites for125 I-labeled melatonin (sup 125 I-melatonin) associated with the microsomal fraction of the rat ventral prostate cells (apparent dissociation constant 0.9 nM).125 I-melatonin binding was inhibited by 2-iodomelatonin> 6-hydroxy-melatonin>melatonin=N-(2,4 dinitrophenyl)-5-methoxytryptamine whereas similar concentrations of serotonin, 5-methoxytryptamine, and tryptamine were less potent. The guanine nucleotide analogs, guanosine 5'-0-[3-thiotriphosphate] and guanosine 5'-0-[2-thio-diphosphate], inhibited specific125 I-melatonin binding whereas 5'-guanylyl imido-diphosphate was less potent.

Conclusions

The results demonstrated putative melatonin receptors in the rat prostate and suggest a direct suppression by melatonin of testosterone-dependent prostate growth.

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