Precise Characterization of Renal Parenchymal Response to Single and Multiple Cryoablation Probes

    loading  Checking for direct PDF access through Ovid


PurposeWe characterized the size and shape of the ablated area, and the surrounding indeterminate zone associated with renal cryolesions produced by single and multiple cryoablation probe configurations in a porcine model.Materials and MethodsLaparoscopic cryoablation, consisting of 2, 10-minute double freeze cycles followed by a 3-minute active thaw, was performed in 18 pigs. Three groups of 6 pigs each were studied, including a single IceRod™, 3 rods arranged in a triangular configuration 2 cm apart from each other and 4 rods arranged in a quadratic configuration 2 cm from each other. Cryoablated kidneys were harvested after 14 days for histopathological evaluation.ResultsThe mean diameter of the area of complete ablation produced by a 1, 3 and 4 rod configuration 1 cm from the tip of the rod was 2.0, 4.4 and 4.9 cm, respectively. Cryolesions were elliptical with 1 cryoablation probe and in the 3 and 4 rod configurations they conformed to triangular and quadratic cross-sectional shapes, respectively. In the zone, ablation a single small focus of peri-arteriolar renal cell viability was noted less than 1 mm from the edge of the cryolesion in 1 of the 18 cryolesions. Otherwise no viable renal cells were detected.ConclusionsMultirod configurations can be used with templates to space cryoablation probes 2 cm apart from each other with reliable and complete ablation overlap between the rods. However, around larger blood vessels the ice ball must be extended to a larger margin to ensure complete tissue ablation.

    loading  Loading Related Articles