The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin

    loading  Checking for direct PDF access through Ovid

Abstract

Objective: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability.

Methods: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8 for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point.

Results: ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4 showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature.

Conclusion: Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature.

Related Topics

    loading  Loading Related Articles