Improved glutathione production by gene expression in Escherichia coli

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Abstract

Aims

To improve glutathione (GSH) production in Escherichia coli by different genetic constructions containing GSH genes.

Methods and Results

GSH production was very low in E. coli by the expression of gshI gene. An increase of GSH production was achieved by the expression of both gshI and gshII genes in E. coli. A higher GSH production, namely 34·8 mg g−1 wet cell weight, was obtained by simultaneous expression of two copies of gshI gene and one copy of gshII gene.

Conclusions

The simultaneous expression of two copies of gshI gene and one copy of gshII gene resulted in a significant increase in GSH production.

Significance and Impact of the Study

The expression strategy for GSH production described here can be used to increase gene expression and obtain high production rates in other multienzyme reaction systems.

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