Improved approach for transferring and cultivatingMethanosarcina acetivoransC2A (DSM 2834)

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Abstract

Aim:

A method for cultivating Methanosarcina acetivorans was further developed to handle these anaerobic archaea without special equipment such as an anaerobic chamber.

Methods and Results:

Medium was filtered and oxygen removed under a nitrogen gas-phase. A dithiothreitol-filled syringe was used to transfer cells from high density grown cultures to new medium. Growth time and cell mass were determined, as well as cell viability was proven by light microscopy.

Conclusion:

Cell transfer and growth was successful using this approach.

Significance and Impact of the Study:

This updated technique allows almost every laboratory the opportunity to grow these methanogenic organisms for further studies. The described method could be used for proteomic analysis and is also interesting for further protein structure determination.

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