Quantitative variability of sugar residues at the surface of Fusarium oxysporum conidia as detected by lectin typing

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G. Recorbet, C. Steinberg AND C. Alabouvette. 1996. Helix pomatia lectin, Concanavalin A, Wheat germ agglutinin and Tetragonolobus purpureas lectin were used to identify N-acetylgalactosamine, D-mannose, N-acetylglucosamine and L-fucose sugar residues, respectively, on the surface of microconidia of Fusarium oxysporum strains. On the basis of the use of FITC-labelled lectins, N-acetylglucosamine, N-acetylgalactosamine and D-mannose sugar residues were identified and did not vary qualitatively between the strains tested. L-fucose residues were not detected, whatever the strain tested. Agglutination experiments showed a quantitative variability in the carbohydrate residues detected with ConA and WGA within the F. oxysporum species. The agglutination responses of the isolates tested did not reflect either their pathogenicity or their host specificity.

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