Assay of bacterial and fungal activity in diesel contaminated soil using a 14C-glucose utilization method

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A.B. Harrison and W.B. Betts. 1996. Changes in the relative metabolism of soil bacteria and fungi following contamination with diesel were assessed using a modified substrate-induced respiration (SIR) method including selective antibiotic inhibition. 14CO2 release from radiolabelled glucose was used as an indication of population activity. In a Sandy Gley Soil with no history of contamination, the population activity shifted from 38 ± 4% (bacterial): 62 ± 4% (fungal) to 73 ± 4% (bacterial): 27±4% (fungal) after treatment with diesel.

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