Galactose transport in Clostridium acetobutylicum P262


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Abstract

Evidence is provided that there are two separate systems for galactose uptake in Clostridium acetobutylicum P262, a phosphoenolpyruvate-dependent phosphotransferase system and a proton motive force-driven system. Glucose uptake involves the former but not the latter. When present on their own in a semi-synthetic medium, glucose and galactose were consumed by the bacterium at rates of 1.00 mmol l-1 h-1 and 0.79 mmol l-1 h-1, respectively. However, when present as a mixture, glucose was used preferentially, and there was a lag period before galactose utilization commenced. The subsequent consumption rates were 0.97 mmol l-1 h-1 and 0.38 mmol l-1 h-1, for glucose and galactose, respectively. These data provide evidence for repression of galactose utilization by glucose.

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