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Forty-seven strains of Campylobacter jejuni were examined by PCR-RFLP analysis of 23S rRNA genes. Seven different molecular profiles were detected by a combination of HpaII AluI and DdeI digest analysis. Most (83%) strains, including those with different Penner serotypes and from different hosts, had the same molecular profiles. The high level of conservation apparent within the 23S rDNA sequences confirmed their value as targets in species-specific PCR identification assays but not for subtypic discrimination within Camp. jejuni.