Sub-typing of animal and human Campylobacter spp. using RAPD


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Abstract

Based on a 10-mer primer (5′-CCTGTTAGCC-3′), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. The method was also successfully applied to typing Campylobacter jejuni from a similar range of sources.

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