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Genetic polymorphism in strains of the entomopathogenic fungus Beauveria brongniartii, isolated from Melolontha melolontha and M. hippocastani in different European sites, was investigated by RAPD-PCR. Strains of B. brongniartii isolated in Valle d'Aosta (Italy) were highly virulent to M. melolontha, and potential relatedness among these strains was investigated with RAPD markers obtained from 16 10-bp primers. This study shows that the strains analysed are closely related. The subdivision of the European strains into subgroups, obtained from numerical analyses of the data, does not appear to relate to either geographical origin or host insect. Individual strain-specific patterns of RAPD bands were identified for 24 strains. The remaining four strains consisted of two pairs, each of which showed identical RAPD patterns.