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The effect of temperature (1-34°C) on the maximum specific growth rate of Aeromonas salmonicida could not be described by the classical growth models; for some strains, two optimal temperatures at 23°C and 30°C were observed, as well as an unexpected increase in the pseudolag time above 27°C. This could be explained by the presence of two subsets, notably S-layer+ and S-layer− sub-populations. The A− cells had higher growth parameters (Topt and μopt) than the A+ cells and were selected by subcultures above 30°C. Yet the relative proportion of A+ cells did not explain all the variation of μmax versus temperature, and the growth kinetics of an Aer. salmonicida isolate remained unpredictable.