Comparison of direct and indirect 'panning' techniques for clarification of Cryptosporidium parvum from aqueous samples


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Abstract

'Panning', a technique developed to separate cells into subpopulations, was evaluated for its potential in recovering Cryptosporidium parvum oocysts from aqueous samples during the clarification stage. Oocysts adhered to the 'pan' by binding to specific anti-oocyst antibody. The sample was clarified by washing off non-adherent debris. Direct and indirect 'panning' trials were conducted. The oocyst recovery rate was approximately 50% for direct panning and 20% for indirect panning. Direct panning resulted in a higher degree of reliability compared with indirect panning. This technique has the potential to be combined with existing or alternative methodologies as a means to clarify oocysts, to allow for a higher degree of oocyst recovery.

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