Use of the indirect immunofluorescence method for detection and enumeration of Escherichia coli in seawater samples

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The determination of Escherichia coli in marine waters through a rapid method, the microscopic indirect immunofluorescent technique, is evaluated in comparison with the conventional count on m-FC agar medium. The data obtained in seawater samples, collected monthly along the Messina coastline, show good sensitivity of the analysis and agreement between the microscopic and culture technique, with a detection limit of 102 cells 100 ml−1 for immunofluorescence.

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