A microwave-based method for nucleic acid isolation from environmental samples


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Abstract

Aims:A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments.Methods and Results:The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction.Conclusions:The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents.Significance and Impact of the Study:The described method can be applied to difficult substrates in environmental microbiology studies.

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