Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris


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Abstract

Aims:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture.Methods and Results:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask culture were found to be 20°C and 6·0, respectively. Sucrose (40 g l–1) and corn steep powder (10 g l–1) were the most suitable carbon and nitrogen source for both mycelial growth and exo-biopolymer production.Conclusions:Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5-l jar fermenter indicated 10·3 g l–1, which was approximately three times higher than that in shake flask culture.Significance and Impact of the Study:This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.

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